11. Charles Bowman, IVF Laboratory Director at Massachusetts General Hospital discusses the advantages of using time-lapse imaging in improving workflow efficiency in IVF laboratories. This is where the Vitrolife Academy steps in - a global network of Vitrolife IVF specialists dedicated to providing clinical support to IVF clinics. RapidVit™ & RapidWarm™ Cleave. Rapid-i vitrification of cleavage stage embryos Vitrification should only be performed by staff trained in vitrification procedures. Rapid-i™ Vitrification Omni. Description G-1™ PLUS is a bicarbonate buffered media containing hyaluronan. On the contrary, spermatozoa undergoing vitrification were healthier regarding morphology with less defects than conventional freezing. (2012) A critical appraisal of cryopreservation (slow freezing versus vitrification) of human oocytes and embryos. Both lasers are calibration free and they can be used for laser applications Vitrolife Multi-Droplet Culture Dish. Sally joined Vitrolife at the beginning of 2019 and before that was at Illumina and prior to that at BlueGnome. She was mentored as an undergraduate and PhD student by David Gardner at Monash University between 1991 to 1997. The oocytes were put in Vitri 1 for 5–20 min; then, they were transferred to Vitri 2 medium for 2–5 min, and then, to the last droplet: Vitri 3. Peer discussions often offer valuable insights. The Rapid-i™ Vitrification System has long been the natural choice for embryologists looking to Different manufacturers of vitrification products have different recommendations regarding procedure temperature, commonly either room temperature or 37⁰C, but which is the optimal temperature for vitrification and why? Dr. In this webinar, Kristina Schildauer, Embryologist and Technical Product Manager at Vitrolife, will compare closed and open carrier devices for vitrification. Due to the density of Vitri 2, the oocytes and embryos will float to the top of the solution. Safety data sheets (SDS) English. Vitrolife is a biotechnology company that develops products to counter fertility issues in humans. Application For use in sequence after equilibration at +37°C and ambient atmosphere. Keep the balance. Thomas Freour will share a retrospective clinical dataset of 7000 warmed blastocysts and describe his experiences with an optimised, closed vitrification system at University Hospital of Nantes, France. Results Cryotop® is the special vitrification container consisting of a fine, thin film strip attached to a hard plastic handle for the minimum volume to realize highest cooling & warming rates resulting in over 90% post-thaw survival. Embryos were placed into the Vitri 1™ Cleave Solution for 5 min, then into the Vitri 2™ Cleave Solution for 2 min, and finally into the Vitri 3™ Cleave Solution for 30 s. Maintaining a defined and physiological pH is through the inclusion of certain chemical components in the media called “pH buffers”. We believe that it is only through our shared knowledge, teamwork and commitment, that we will be able to ensure sustainability in everything we do and become even more successful. Hereby, the compatibility of vitrification Kit 1 with warming Kit 2 was also examined. The opinions expressed by Vitrolife’s guest bloggers are their own and do not necessarily reflect Vitrolife's opinions or views. In this movie he demonstrates how to u 7000 warmed blastocysts in 7 years - Experiences with an optimised closed vitrification system In this recorded webinar, Prof. Vitrification and warming were carried out at physiological Vitrification online training course. In vitro, the pH is generally maintained between 7. The goal is to ensure the maximum survival rate with the highest level of biosafety. For detailed instructions, consult the Vitrification has in the recent years become the cryopreservation method of choice in many IVF-clinics. Jul 6, 2017 · By Vitrolife, Jul 6, 2017 How to improve workflow efficiency and maximise clinical outcomes in your IVF clinic [recorded symposium] During the Vitrolife Scientific Symposium at ESHRE 2017, we had the pleasure of listening to three international experts in the field of human IVF sharing their experience in laboratory management, morphokinetics G-IVF™ PLUS. Rapid-i Vitrification System - Tips & Tricks This guide highlights practical tips and tricks for successful vitrification. In this presentation, Dr. com . By Vitrolife , Jan 10, 2023. Application For use in sequence after equilibration at room temperature and ambient atmosphere. Validation and clinical implementation of a fast, standardised and closed vitrification system for human oocytes. Avoid ice formation. 4%; Vitrolife: 87. 2 and 7. com May 2, 2022 · A part of the series "6 Tips & Tricks for the Rapid-i Vitrification System" by Vitrolife. Ann-Helene Jakobsson will share insights about oocyte vitrification. Garnder scoring system were used to evaluated blastocyst morphology based on the inner cell mass and trophectoderm grading [ 12 ]. Minimum toxic exposure. Dec 7, 2018 · Time-efficient, easy and successful vitrification for all cell stages. This presentation was part of Vitrolife and Igenomix live Academy Studios from ASRM 2023. Welcome to Vitrolife - Vitrolife Oct 11, 2022 · Vitrolife Smartbox - Designed to keep you organised and safe. The company also develops various media that enable researchers and cell therapy companies to use cells for therapeutic purposes. You will learn about the challenges with oocyte vitrification, how to optimise your workflow and save time in every procedure and get practical tips and tricks on how to succeed with Rapid-i Vitrification System. Topics: Vitrification , IVF Downloads. The development of the Rapid-i™, a closed vitrification device that does not use direct contact with liquid nitrogen during vitrification or subsequent storage, will be discussed as well as clinical protocols for human oocytes and embryos. Dec 22, 2022 · 2. If you want to have a more practical, hands-on experience and get trained in specialist techniques, this is the workshop for you! You will learn about the fundamental principles of blastocyst culture, biopsy, genetic diagnosis and vitrification. After over a decade on the market, the Oocyte retrieval needles and gynaecological needles by Vitrolife. Another important parameter is the temperature at which vitrification is taking place. Dr. The How to save time and secure good results in vitrification [webinar recording] Maintaining correct temperature in the culture system [customer story] Thoughts and reflections from ESHRE 2017 Our dishes are specifically designed to support and facilitate the various procedures during the IVF process to make your way of working easier and more effective. The following is the general procedure for using RapidVit™ Blast. Version 02. Easy to use – for all cell stages. Kitazato, world leader in vitrification, has developed The Cryotop® Method and secured its global implementation achieving best-in-class results in cryopreservation of human specimens from gametes to blastocysts. Ann-Helene Jacobsson has summarised five published studies that discuss the optimal temperature for vitrification. Dec 9, 2021 · One study compared the effects of the Irvine vitrification kit and the Vitrolife vitrification kit on clinical outcomes after the cryopreservation of cleavage-stage embryos and suggested that the two methods yielded similar clinical outcomes; however, the study focused only on the embryo implantation rates and did not investigate the effects on Vitrification Media. Mark Larman from Vitrolife talks about the need for closed vitrification systems and the development of Rapid-i Vitrification System from Vitrolife. Density gradient centrifugation using SpermGrad (Vitrolife) enrich for sperm with high mobility and low DNA fragmentation and is the preferred technique for selection of sperm with longer telomeres. Vitrolife is a highly regarded and widely recognized brand in the IVF industry, known for its exceptional quality and reliability. 3. 5, 6. SpermGrad™. Vitrolife 5-well culture dish Rapid-i™ Sealer Rapid-i™ CryoCane Rapid-i™ Goblet Fill the SmartBox with liquid nitro-gen up to 1 cm from the box’s rim and place the lid on top of the box. Select and support the most viable sperm. Precision and Consistency in Embryo Vitrification and Storage. Sally Cartwright. You will learn more about the details surrounding the Vitrolife media and devices used during vitrification and warming as well as the associated protocols. They showed that live birth rates from vitrified slow developing (compacting) embryos on day 5 as well as poor-quality blastocysts contribute substantially to overall success rates. Conventional freezing method resulted in better motility, viability in both washed/unwashed groups. Gx-IVF has triple antioxidant protection for improved embryo viability. vitrolife. Visit . However, most of these Dec 5, 2022 · Background Indications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. Hashimoto works at IVF Namba Clinic in Osaka, Japan and has published several scientific studies on vitrification. Kurkkio and Bohlin 2022. . 2. We provide products of superior quality to IVF clinics worldwide. With survival rates superior to slow freezing and developmental potential of oocytes and embryos similar to fresh ones, results similar to fresh oocytes and embryos can be 30 years of Innovations. The new generation of slow freezing – optimised composition, convenient protocol and high survival rate. This unique innovation takes culture in micro-droplets to a higher level. Jun 13, 2023 · Kit 1 (RapidVit™ Blast/RapidWarm™ Blast, Vitrolife, Sweden) was validated in 2010, whereas Kit 2 (RapidVit™ Omni/RapidWarm™ Omni, Vitrolife, Sweden) was validated in 2016. The dish has a security ledge. In this recorded webinar Dr. 2. Vitrification Place 0. SDS SpermGrad. Workshop Vitrolife 5-well culture dish Rapid-i™ Sealer Rapid-i™ CryoCane Rapid-i™ Goblet Fill the SmartBox with liquid nitro-gen up to 1 cm from the box’s rim and place the lid on top of the box. Created after four years of intensive development, Gavi® is the first technology to automate the critical equilibration step, helping to standardise the complex vitrification process. Luyet, the so-called Father of Cryobiology (Luyet, 1937). FreezeKit™ & ThawKit™ Cleave. Gavi® approaches vitrification in an entirely new way, helping to achieve consistent high-quality Oct 15, 2021 · This work will be presented in more detail in our 2022 Sustainability Report. 8%) (p < 0. Presenter - Dr. The α-numeric identification secures and facilitates embryo identification, both when viewed in and outside of the microscope. What is the one thing you wish every embryologist would know about vitrification? We asked Hubert Joris, Media Product Development Manager at Vitrolife Group, about the development within Oct 20, 2021 · Since the beginning of IVF and cryostorage of cells in general, different ways to monitor the contents of our liquid nitrogen (LN2) dewars have been developed. Methods Patient demographic, embryo, and cycle characteristics associated with failed euploid blastocyst survival were compared in a cohort of women (n = 6167) who underwent IVF-PGT-A. Workshop: 'Blastocyst culture, biopsy and vitrification' in partnership with Embryotools. Important factors for successful vitrification Vitrification has in the recent years become the cryopreservation method of choice in many IVF-clinics. Maximum embryo support. Feb 24, 2024 · The embryos transferred to G2 medium (Vitrolife, Sweden) were cultured to the blastocyst stage for fresh embryo transfer or vitrification. Additionally, albumin can protect embryos from toxicity in the culture environment, act as a chelator and pH buffer (Gardner, 2008). , (P-135) using the Vitrolife vitrification system, presented results challenging current practices in blastocyst selection criteria. RapidVitTM Blast and RapidWarMmM Blast is similar to the predicate device in most respects. Embryos vitrified using a KITAZATO Vitrification Kit G-1™ PLUS provides the carbohydrates, amino acids and hyaluronan needed to support the embryo during the first critical stages of development. Read more about the optimal temperature for To avoid cell damage and unnecessary stress to oocytes, short defined time frames at 37⁰C should be used for vitrification and warming to achieve optimal survival rates and subsequent embryo development. For detailed instructions, consult the Succeed with vitrification - 3 factors that will influence your result. No differences in survival rates were observed between the two vitrification methods, but a significant higher cleavage rate was observed using Irvine compared to Vitrolife method (p < 0 Jun 22, 2017 · With our Rapid-i Vitrification System you get time-efficient, easy and successful vitrification for all cell stages. Most vitrified embryos will survive the vitrification-warming process [ 8 ]. Vitrolife sequential media versus Irvine single step media systems for the culture and selection of human embryos for transfer and vitrification Previous Article Effects and pregnancy outcomes of L-carnitine supplementation in culture media for human embryo development from in vitro fertilization (IVF) Aug 19, 2013 · Dr. Vitrification has in recent years become the cryopreservation method of choice in many IVF clinics. RapidVitTM Summary of Safety & Clinical Performance. Nov 28, 2018 · The many faces of albumin and its vast history in IVF. At Vitrolife, all colleagues contribute to our success. Ensure Cleave Numerous publications show that Rapid-i Vitrification System provides excellent . Albumin in the culture media acts as a surfactant, preventing embryos and gametes from sticking to consumables due to changes in the surface charge. Feb 12, 2020 · It is with great sadness that Vitrolife posts this memoriam for Prof Michelle Lane, who passed away last week. G-IVF Plus is a fertilisation medium for handling and preparation of gametes. Nov 18, 2019 · Instruction video for oocyte vitrification. Apr 19, 2017 · All of Vitrolife’s culture and vitrification/warming media contain hyaluronan, which has been shown to increase cryotolerance [14–18]. The applied vitrification procedure combines ultra rapid cooling/warming with high cryoprotectant concentrations and minimal volume methods [29–31]. Find CoA’s, technotes, package inserts, SDS’s and other documents supporting our products. With survival rates superior to slow freezing and developmental potential of oocytes and embryos similar to fresh ones, results similar to fresh oocytes and embryos can be obtained. The dish is equipped with 12 micro-wells optimised for 25-35 µL droplets. Successful embryo cryopreservation is an important step for IVF laboratories to maximize a patient's IVF cycle. Post-warming embryos survival rate was significantly higher after vitrification (Irvine: 89. | Vitrolife is committed to supporting successful Vitrolife Sweden AB for the vitrification of day 3 cleavage stage embryos. Vitrolife Method Protocol . This is useful to determine if a top-up is required, however this Feb 1, 2018 · Several authors have reported that artificial shrinkage before vitrification of blastocysts improves survival rate and increases the clinical success of blastocyst cryopreservation programmes (Darwish, Magdi, 2016, Hiraoka et al, 2004, Iwayama et al, 2011, Mukaida et al, 2006, Son et al, 2003, Vanderzwalmen et al, 2002). Storage Store dark at +2 to +8°C. By Vitrolife, Jan 27, 2022 The concept of fast freezing or vitrification was first described more than 80 years ago by Basile J. The concept of fast freezing or vitrification was first described more than 80 years ago by Basile J. 5-1 ml of each of the following media By Vitrolife , Jan 27, 2022. Human Reproduction Update, 18, 536-554. Ann-Helene Jakobsson Dec 8, 2013 · Vitrolife vitrification procedure and media (Vitrolife group) The entire procedure was performed at 37 °C according to the manufacturer’s instructions. Luyet , the so-called Father of Cryobiology (Luyet, 1937). The inclusion criterion for the study was donors providing a minimum of 12 MII oocytes at retrieval. from $91. Sally has significant experience in managing products in the market and taking news We offer a comprehensive range of premium-quality products, including ovum pick-up sets, vitrification equipment, media and micromanipulation tools, tailored to the needs of registered and approved fertility centers, medical facilities, animal IVF, and research centers. If denu-dation and fertilisation assessment can be performed within 2 minutes, G-IVFTM PLUS can be used instead of. Vitrolife data-on-file, REP-4336-v. IVF media and oil for optimal handling and culture in the IVF procedure, all the way from preparation to embryo transfer. vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses. Jan 25, 2013 · A consensus has slowly emerged stating that vitrification procedures result in a better embryological and clinical outcome than the slow-freezing procedures [9, 12, 18, 24, 34, 35, 42, 55, 56]. The ramp makes for easier access to embryos as they are clearly visible when at the periphery of the wall. 14. RapidVit and RapidWarm Omni have been developed for vitrification and warming of all cell stages, from the oocyte through to the blastocyst stage, and using the same media with cell-specific timings. Description MOPS buffered media. There are many studies showing that vitrification at 37⁰C is the optimal temperature in order to not damage the spindle and to achieve good results. Vitrolife MicroCell. With a focus on making the dream of parenthood a reality, Vitrolife is committed to providing the best-in-class products and services for every stage of the IVF journey. Learn how to perform vitrification and warming using RapidVit Omni and RapidWarm Omni Vitrolife | 22,986 followers on LinkedIn. However, the predicate device RapidVitM Cleave and Rapid WarMTM Cleave (K080446) is intended for vitrification of day 3 cleavage stage embryos. Learn more at vitrolife. G-MOPS PLUS. Purpose Medium for culture of embryos from the pronucleate stage to day 2 or day 3. Mar 30, 2011 · The open versus closed vitrification systems did not overtly affect the degree of cryo-injury. 6%) than after slow freezing (63. 4. 0001). Sally has a PhD Genetics and has worked in the US and Germany. In this study, we evaluated the efficiency of the semi-automated technique of oocyte vitrification by survival rate, morphometric assessment and resistance to Jul 26, 2021 · Purpose To understand the clinical factors associated with embryo survival after vitrification in a cohort of human blastocysts screened by preimplantation genetic testing for aneuploidy (PGT-A). 41. outcomes after vitrification of all stages, from oocytes to blastocysts. Vitrolife was founded in 1994 and is based in Frolunda, Sweden with subsidiaries in USA, Hungary, Australia, France, The Cryotop® Method. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. Sperm processing when accuracy, performance and convenience matters. Both vitrification and warming are performed at physiological temperature (37°C). Since 2004, Sally has been living and working in Cambridge, UK. Edgar, D. Different manufacturers of vitrification products have different recommendations regarding procedure temperature, commonly either room temperature or 37⁰C, but which is the optimal temperature for vitrification and why? Dr. Oocyte vitrification: clinical Vitrolife 5-well culture dish Rapid-i™ Sealer Rapid-i™ CryoCane Rapid-i™ Goblet Fill the SmartBox with liquid nitro-gen up to 1 cm from the box’s rim and place the lid on top of the box. Data was collected retrospectively (Jan 2018 – Dec 2020) following 3 004 transfers from two clinics in the USA. After warming, blastocysts were cultured in G-TL™ medium (Vitrolife) supplemented with 20% HSA-solution™ (Human Serum Albumin) for 2 h before the transfer. Keywords: Closed device; Contamination; Cryopreservation; Liquid nitrogen; Rapid-i; Vitrification. 47. A common method is the use of a level ruler to determine the level of LN2 inside the dewar at a specific point in time. Implementation deadlines for existing devices are dependent on classification and expiry of existing MDD certificates. FUJIFILM Irvine Scientific’s vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation programs that are delivering babies every day. procedure before using Vitrolife RapidVit™ Blast and RapidWarm™ Blast kits for vitrification and warming of human blastocyst stage embryos. Webinar: 'Workflow efficiency with Time-lapse'. However, it has been shown that between 1 and 5% of Downloads. Keep the correct speed. View documents. 0 Design Validation Report - Rapid-i Kit. He showed that supercooled solutions could be solidified without crystallization, forming a glass-like state. 37°C. Michelle contributed significantly to the field of IVF for 30 years. A Rapid-i™ (Vitrolife, Sweden) was used as carrier and storage device. Our dedicated team of specialists is devoted to offering personalized Jan 1, 2008 · Good quality blastocysts were vitrified on D5 or D6 using Irvine Vitrification medium (Irvine Scientific-USA) and the Rapid-I closed Vitrification System™ (Vitrolife, Sweden). Ensure While the Vitrolife product portfolio is designed for comprehensive utility, there may be times when customisation is beneficial. All Vitrolife media are formulated to meet this narrow pH specification range, supporting optimal metabolic conditions. H. Working together with us means having a partner that understands the challenges involved in running an IVF clinic. Oct 14, 2015 · 2. 12. Purpose For vitrification and warming of cleavage stage embryos (US: day 3 only). The oval centre well allows for easy instrument access from the sides. May 22, 2019 · Metaphase II (MII) oocytes were incubated at 37°C in 6% CO 2 and 95% air in a specific medium (IVF®, Vitrolife, Göteborg) until vitrification was performed. It also contains human serum albumin. Note that the timing has to be accurate. For more educational material such as instruction movies, blog posts, short protocols, clinical evidence and much more. Already then, the potential of the technique Rapid-i Vitrification System - Tips & Tricks This guide highlights practical tips and tricks for successful vitrification. From this date, all new medical devices and the manufacturer’s quality management system must comply with the MDR. Rapid-i vitrification Omni For all stages, from oocytes to blastocysts Vitrification should only be performed by staff trained in vitrification Vitrification has evolved into a reliable and efficient method to freeze oocytes and embryos. In this recorded webinar, Kristina Schildauer, Embryologist and Technical Product Manager at Vitrolife, will compare closed and open carrier devices for vitrification. Download Table | Summarised results of cryopreserved/warmed embryos by vitrification Irvine, vitrification Vitrolife and slow-freezing methods (IR implan- tation rate; LB live birth rate) from Jul 26, 2021 · Compared to slow-freeze protocols, vitrification has been shown to result in higher embryo survival rates and implantation and pregnancy rates, at both the cleavage stage [ 3, 4] and blastocyst stage [ 5 – 9 ]. Guaranteed embryo and ga. For three decades, Vitrolife has been at the forefront of innovation and pioneered the field of assisted reproduction. Vitrolife Labware - clinical evaluation and user experiences. Dec 8, 2013 · Vitrolife vitrification procedure and media (Vitrolife group) The entire procedure was performed at 37 °C according to the manufacturer’s instructions. Date of issue 220923. Rapid-i vitrification Omni For all stages, from oocytes to blastocysts Vitrification should only be performed by staff trained in vitrification Sep 22, 2021 · Eventually, the new Medical Device Regulations (MDR) were developed and came into force on May 26, 2021. This study confirmed the efficacy of applying a single warming protocol, despite what the "industry" has led us to believe, supporting the idea that it is time to proceed in the cryopreservation field and encouraging embryologists worldwide to come out and reveal that such a procedure is possible an … Aug 20, 2020 · Gunst et al. Vitrification was conducted using Vitrolife vitrification media and Rapid-I devices (Vitrolife) at a temperature of 37 °C according to the manufacturer’s protocol. Make a difference for your patients. 51. As a leading partner, we have earned the trust of clinics and patients globally. The small diameter of the bottom facilitates easy embryo location. For all stages, from oocytes to blastocysts. Remove cumulus and corona cells from oocytes using a denudation pipette and assess fertilisation at. For ICSI oocytes, assess fertilisation in the G-1 PLUS micro-droplet culture dish. Document and Ref: Date Revised : 06/27/2023. In addition, 37⁰C should also be a safer procedure when considering the potential risk of epigenetic effects after cryopreservation. By Vitrolife , Jan 27, 2022. Our objective is to provide you a method with proven evidence of success and to help you obtain the best Apr 12, 2023 · Purpose The purpose of this study is to assess the impact of different temperatures and incubation times on the clinical outcomes of FET cycles during the thawing procedure and to select a better thawing method to improve clinical outcomes. However, there was a significantly higher rate of DNA damage after vitrification at the blastocyst stage compared to cleavage stage embryos (P < 0. 001). The percentage of blastomeres per embryo showing DNA damage was similar between carriers. The static Octax LaserShot M and the dynamic Octax NaviLase provide excellent image quality and safe procedures. Document and Ref: REF 19105. This short protocol describes how to vitrify and warm all cell stages from oocytes to blastocysts using Rapid-i ™vitrification system and RapidVit™/Warm™ Omni. A closed device (Rapid-i™ Kit) and MOPS buffered media (RapidVit™ Blast, RapidWarm™ Blast) were used for vitrification and warming of 3 491 blastocysts. Tubing, gel and covers to facilitate the oocyte retrieval process. Learn how to perform oocyte vitrification using RapidVit Oocyte and RapidWarm Oocyte from Vitrolife Dec 22, 2022 · Vitrification was conducted using Vitrolife vitrification media and Rapid-I devices (Vitrolife) at a temperature of 37 °C according to the manufacturer’s protocol. The Cryotop® Method is simple, reliable, universal safe and easy for anyone. Revision No : 5. The finished products are further tested to offer a secure environment for gametes and embryos. Vitrolife IVF Certified Culture Dish 40mm Like the other dishes intended for culture, this dish has tapered edges. Rapid-i vitrification of blastocysts Vitrification should only be performed by staff trained in vitrification procedures. Oct 23, 2013 · Dr. Sperm washing before frozen was a beneficial preparation to sper … Octax is more than just a laser; it is a multipurpose platform for digital imaging, image analysis and advanced applications in assisted reproduction. Vitrolife dishes are manufactured with quality controlled and certified materials. Log in to purchase. You will also get an insight into liquid nitrogen-mediated transmission of infectious agents and health authorities’ views of the different devices. Methods This retrospective study included 1734 FET cycles from January 1, 2020, to January 30, 2022. 5. Vitrolife IVF Certified Center Well Dish The Centre Well Dish is a multi-purpose dish for fertilisation, cryo-procedures, embryo culture and embryo transfer. and Gook, D. Description MOPS buffered media containing cryoprotectants and human serum albumin. 00. A. This course cover the background and technical details surrounding the vitrification and warming of oocytes and embryos. After a post-doc with Barry Bavister in Wisconsin Vitrolife 5-well culture dish Rapid-i™ Sealer Rapid-i™ CryoCane Rapid-i™ Goblet Fill the SmartBox with liquid nitro-gen up to 1 cm from the box’s rim and place the lid on top of the box. ICSI is designed for the immobilization and isolation of sperm prior to intracytoplasmic sperm injection, ICSI. go to xa et zu ul lw wh kn dm